|Worm gene name:||KO2D7.1|
|Worm sequence name:||K02D7.1|
|Related human gene:||NP|
|Associated human disease:||Deficiency of nucleoside phosphorylase resulting in defective T-cell immunity|
|People involved in this project:||
|Left primer sequence:||tttagtgcaccctcccagtc|
|Right primer sequence:||ggcaagttgacattccgagt|
|Size of PCR product:||861|
|Brief description:||the gene has been inserted into L4440 and transformed into DH5-alpha E.coli cells. I have also plated this transformed cells for future studies under the label "#21", "DH5-alpha", K02D7.1", "Joseph". dated 7/26.|
|Report any problems that might have appeared and any solutions:|| When i used the competent cells that have been prepared, i couldn't get any colonies after the transformation.
When I used the competent cells which I prepared on my own, there were colonies.