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Worm gene name:  rnt-1
Worm sequence name:  B0414.2
Related human gene:  RUNX1
Associated human disease:  Acute Myeloid Leukemia
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Left primer sequence:  tattatccgaacgcctggac
Right primer sequence:  cgaggagaagatggtcgttt
Size of PCR product:  1011
Brief description:  The protein encoded by the human gene represents the alpha subunit of Core Binding Factor (CBF) and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Mutations in RUNX1 can cause autosomal dominant familial platelet disorder associated with development of acute myelogenous leukemia. To study this platelet disorder in a model organism, I searched for related genes in C. elegans by using BLAST and found that rnt-1 was the most closely related gene to RUNX1 in worms. I then designed primers to amplify the gene and transform into an RNAi feeding strain of E. coli. At this time no tests have been done looking at what happens when the worms ingest the RNAi with rnt-1.
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