Worm gene name:  mrp-6
Worm sequence name:  F20B6.3
Related human gene:  CFTR
Associated human disease:  Cystic Fibrosis
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Left primer sequence:  ttcgggcgtgaactattttc
Right primer sequence:  ttgagcacaataagcgaacg
Size of PCR product:  507
Brief description:  Source: OMIM
Cystic fibrosis transmembrane conductance regulator (CFTR) functions as a chloride channel and controls the regulation of other transport pathways. Mutations in the CFTR gene have been found to cause cystic fibrosis and congenital bilateral aplasia of the vas deferens.
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I tried to use the primers to make an amplicon for cloning into L4440. The PCR at both 55 and 50*C did not give any product.
Posted by Virginia Balke on 2008-08-22 13:30:12